cat 130 091 041 Search Results


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Miltenyi Biotec s1 peptide pool
Fig. 4. Cellular immune response to pVAC15, 16 and 17 at 16 months post vaccination. (A) Detection of IFN-γ responses in Balb/c mice post administration of DNA vaccine pVAC15, 16 and 17. T cell responses were measured by IFN-γ ELISpot in spleenocytes stimulated for 20 h with overlapping peptide pools spanning SARS-CoV-2 spike, <t>S1</t> region. The phenotype of the cellular response was measured by flow cytometry. (C) CD4 and (D) CD8 T cells expressing IFN-γ and TNF-a following in vitro spike stimulation are presented. *P < 0.05, **P < 0.01, **** P < 0.0001.
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Miltenyi Biotec human cd31 microbead kit
Fig. 4. Cellular immune response to pVAC15, 16 and 17 at 16 months post vaccination. (A) Detection of IFN-γ responses in Balb/c mice post administration of DNA vaccine pVAC15, 16 and 17. T cell responses were measured by IFN-γ ELISpot in spleenocytes stimulated for 20 h with overlapping peptide pools spanning SARS-CoV-2 spike, <t>S1</t> region. The phenotype of the cellular response was measured by flow cytometry. (C) CD4 and (D) CD8 T cells expressing IFN-γ and TNF-a following in vitro spike stimulation are presented. *P < 0.05, **P < 0.01, **** P < 0.0001.
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Beijing Solarbio Science polybrene solarbio
Fig. 4. Cellular immune response to pVAC15, 16 and 17 at 16 months post vaccination. (A) Detection of IFN-γ responses in Balb/c mice post administration of DNA vaccine pVAC15, 16 and 17. T cell responses were measured by IFN-γ ELISpot in spleenocytes stimulated for 20 h with overlapping peptide pools spanning SARS-CoV-2 spike, <t>S1</t> region. The phenotype of the cellular response was measured by flow cytometry. (C) CD4 and (D) CD8 T cells expressing IFN-γ and TNF-a following in vitro spike stimulation are presented. *P < 0.05, **P < 0.01, **** P < 0.0001.
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Fig. 4. Cellular immune response to pVAC15, 16 and 17 at 16 months post vaccination. (A) Detection of IFN-γ responses in Balb/c mice post administration of DNA vaccine pVAC15, 16 and 17. T cell responses were measured by IFN-γ ELISpot in spleenocytes stimulated for 20 h with overlapping peptide pools spanning SARS-CoV-2 spike, S1 region. The phenotype of the cellular response was measured by flow cytometry. (C) CD4 and (D) CD8 T cells expressing IFN-γ and TNF-a following in vitro spike stimulation are presented. *P < 0.05, **P < 0.01, **** P < 0.0001.

Journal: Vaccine

Article Title: Strong immunogenicity & protection in mice with PlaCCine: A COVID-19 DNA vaccine formulated with a functional polymer.

doi: 10.1016/j.vaccine.2024.01.065

Figure Lengend Snippet: Fig. 4. Cellular immune response to pVAC15, 16 and 17 at 16 months post vaccination. (A) Detection of IFN-γ responses in Balb/c mice post administration of DNA vaccine pVAC15, 16 and 17. T cell responses were measured by IFN-γ ELISpot in spleenocytes stimulated for 20 h with overlapping peptide pools spanning SARS-CoV-2 spike, S1 region. The phenotype of the cellular response was measured by flow cytometry. (C) CD4 and (D) CD8 T cells expressing IFN-γ and TNF-a following in vitro spike stimulation are presented. *P < 0.05, **P < 0.01, **** P < 0.0001.

Article Snippet: Each spleen was then stimulated with S1 peptide pool (10-15mers with 11 aa overlap, Miltenyi Cat, #130–127-041) at a final conc of 1 μg/mL for 18–20 h along with nonstimulated negative controls.

Techniques: Enzyme-linked Immunospot, Flow Cytometry, Expressing, In Vitro